Flora and fauna of the area is consistent with that which is generally associated with front-range biotic communities from the Palmer Divide on the south to the Wyoming border on the north. Slopes at the Balarat site support ponderosa pine or Douglas fir trees, depending upon the site aspect. Aspen groves will be found mixed in with the north facing fir populations and aspen is also common along the riparian areas of the site. A relic population of limber pine is found at the main entrance and in a few other locations as well. Blue spruce and lodgepole pine are associated with a cooler, wetter environment on the east side of the Balarat site and an area immediately east of the eutrophic pond is home to a particularly interesting population of dwarf and misshapen ponderosa pine.

Balarat meadows contain grasses native to the west such as buffalo and gramma grass. A wide variety of perennial herbs and shrubs occupy the site.